Ready-to-Assay SST4 Somatostatin Receptor Frozen Cells

Background Information:

The Ready-to-Assay SST4 Somatostatin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. somatostatin) based activation of SSTR4 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress SSTR4 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of SSTR4 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Somatostatin

Sub Family:

Class A

Protein Name:

sst4

Protein Aliases:

SS4-R|SS4R

Accession Number:

AY548169

UniProt Number:

P31391

Gene Name:

SSTR4

Gene ID:

6754

Target Species:

Human

Cell Background:

Chem-1