Ready-to-Assay PK2 Prokinecticin Receptor Frozen Cells

Background Information:

The Ready-to-Assay PK2 Prokineticin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. Prokineticin, EG-VEGF) based activation of PROKR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PROKR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PROKR2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Prokineticin

Sub Family:

Class A

Protein Name:

PK2

Protein Aliases:

G-protein coupled receptor 73-like 1|G-protein coupled receptor I5E

Accession Number:

NM_144773

UniProt Number:

Q8NFJ6

Gene Name:

PROKR2

Gene ID:

128674

Gene Aliases:

GPR73b|PKR2|GPRg2|dJ680N4.3|GPR73L1

Target Species:

Human

Cell Background:

Chem-1