Ready-to-Assay PK1 Prokinecticin Receptor Frozen Cells

Background Information:

The Ready-to-Assay PK1 Prokineticin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. Prokineticin, EG-VEGF) based activation of PROKR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PROKR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PROKR1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Prokineticin

Sub Family:

Class A

Protein Name:

PK1

Protein Aliases:

G-protein coupled receptor 73|G-protein coupled receptor ZAQ

Accession Number:

NM_138964

UniProt Number:

Q8TCW9

Gene Name:

PROKR1

Gene ID:

10887

Gene Aliases:

PKR1|ZAQ|GPR73a|GPR73

Target Species:

Human

Cell Background:

Chem-1