Ready-to-Assay NPBW1 Neuropeptide B/W Receptor Frozen Cells

Background Information:

The Ready-to-Assay NPBW1 Neuropeptide B/W Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. neuropeptide B/W) based activation of NPBWR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress NPBWR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of NPBWR1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Neuropeptide W / Neuropeptide B

Sub Family:

Class A

Protein Name:

NPBW1

Accession Number:

NM_005285

UniProt Number:

P48145

Gene Name:

NPBWR1

Gene ID:

2831

Gene Aliases:

GPR7

Target Species:

Human

Cell Background:

Chem-4