Ready-to-Assay MRGPRX2 Receptor Frozen Cells

Background Information:

The Ready-to-Assay MRGPRX2 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. cortistatin-14, PAMP) based activation of MRGPRX2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress MRGPRX2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MRGPRX2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Class A Orphan

Sub Family:

Orphan and other 7TM

Protein Name:

MRGX2 (MRGPRX2)

Accession Number:

NM_054030

UniProt Number:

Q96LB1

Gene Name:

MRGPRX2

Gene ID:

117194

Gene Aliases:

MRGX2

Target Species:

Human

Cell Background:

Chem-1