Ready-to-Assay LH Glycoprotein Hormone Receptor Frozen Cells

Background Information:

The Ready-to-Assay LH Glycoprotein Hormone Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. [ligand]) based activation of LHCGR GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress LHCGR alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of LHCGR , the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Glycoprotein Hormone

Sub Family:

Class A

Protein Name:

LH

Protein Aliases:

Luteinizing hormone receptor|LHR|LSH-R

Accession Number:

BC156303

UniProt Number:

P22888

Gene Name:

LHCGR

Gene ID:

3973

Gene Aliases:

LHR|LCGR|LGR2|ULG5|LGR2|LHRHR

Target Species:

Human

Cell Background:

HEK293