Ready-to-Assay CCR3 Chemokine Receptor Frozen Cells

Background Information:

The Ready-to-Assay CCR3 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. TARC, MDC) based activation of CCR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CCR3 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR3 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Protein Name:

CCR3

Protein Aliases:

CC-CKR-3|CCR-3|CCR3|CKR3|Eosinophil eotaxin receptor|CD antigen CD193

Accession Number:

U28694

UniProt Number:

P51677

Gene Name:

CCR3

Gene ID:

1232

Gene Aliases:

CC-CKR-3|CKR3|CD193

Target Species:

Human

Cell Background:

Chem-1