Biological Information

Background Information:

The PathHunter® MC1R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. α-MSH) based activation of MC1R GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged MC1R and EA-tagged β-Arrestin 2. Activation of MC1R stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Melanocortin

Sub Family:

Class A

Coupling:

Gs

Protein Name:

MC1

Protein Aliases:

alpha melanocyte stimulating hormone receptor

Accession Number:

NM_002386

UniProt Number:

Q01726

Gene Name:

MC1R

Gene ID:

4157

Gene Aliases:

MSH-R|MSHR

Target Species:

Human

Cell Background:

U2OS

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®