PathHunter® SSTR2 Activated GPCR Internalization CHO-K1 Cell Line

Background Information:

The PathHunter® SSTR2 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. somatostatin 28) based internalization of SSTR2 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged SSTR2. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting SSTR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Somatostatin

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

sst2

Protein Aliases:

SS2-R|SS2R|SRIF-1

Accession Number:

AY236542

UniProt Number:

P30874

Gene Name:

SSTR2

Gene ID:

6752

Target Species:

Human

Cell Background:

CHO-K1