PathHunter® Rhesus TrkA-P75 Functional Kinase Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Rhesus TrkA-P75 Functional Kinase Cell-Based Assay Kit (U2OS) utilizes the U2OS rhesus TrkA-rhesus P75 Functional Assay Cell Line. This cell based assay is developed to study drug candidates (small molecules or biologics) targeting receptor tyrosine kinases (RTKs). This kit assesses RTK activation by detecting the recruitment of SH2 domain proteins to activated rhesus TrkA-p75 dimers. The included cell line overexpresses PK-tagged rhesus TrkA, untagged rhesus P75, and an EA-tagged SH2 domain protein. Upon ligand (e.g. βNGF)-induced receptor activation, SH2-EA recruitment leads to the production of an EFC signal proportional to the degree of receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

TRKA

Protein Aliases:

TRK1-transforming tyrosine kinase protein|Tropomyosin-related kinase A|Tyrosine kinase receptor|Tyrosine kinase receptor A|Trk-A|gp140trk|p140-TrkA|high affinity nerve growth factor receptor

Accession Number:

XM_001114243.2

UniProt Number:

F7DJ75

Gene Name:

NTRK1

Gene ID:

719670

Gene Aliases:

TRK|TRKA|MTC

Target Species:

Rhesus Macaque

Cell Background:

U2OS