PathHunter® P2RY2 Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® P2RY2 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. UTP, ATP) based internalization of P2RY2 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged P2RY2. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting P2RY2-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

P2Y

Sub Family:

Class A

Coupling:

Gq

Protein Name:

P2Y2

Protein Aliases:

ATP receptor|P2U purinoceptor 1|P2U1|P2U receptor 1|Purinergic receptor

Accession Number:

AY136753

UniProt Number:

P41231

Gene Name:

P2RY2

Gene ID:

5029

Gene Aliases:

P2U

Target Species:

Human

Cell Background:

U2OS