PathHunter® Human TACR2 β-Arrestin Cell-Based Assay Kit (CHO-K1)

Background Information:

The PathHunter® Human TACR2 β-Arrestin Cell-Based Assay Kit (CHO-K1) utilizes the Human TACR2 β-Arrestin cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of β-arrestin recruitment following receptor activation for GPCR drug discovery and development programs. This cell line enables the assessment of ligand (e.g., substance P) based activation of TACR2 GPCR activity via detection of β-arrestin recruitment. This cell line employs Enzyme Fragment Complementation (EFC) technology that combines an overexpressed PK-tagged TACR2 target receptor and an EA-tagged β-Arrestin 2 to generate a chemiluminescent signal proportional to receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Tachykinin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

NK2

Protein Aliases:

NK-2 receptor|NK-2R|Neurokinin A receptor|Tachykinin receptor 2

Accession Number:

AY322545

UniProt Number:

P21452

Gene Name:

TACR2

Gene ID:

6865

Gene Aliases:

SKR|NK2R|NKNAR|TAC2R

Target Species:

Human

Cell Background:

CHO-K1