PathHunter® Human PDGFRb Stable Cell Line Functional Kinase Assay (U2OS)

Background Information:

The PathHunter Human PDGFRb Stable Cell Line Functional Kinase Assay (U2OS) utilizes the U2OS PDGFRb Functional Assay Cell Line. This cell based assay is developed to study drug candidates (small molecules or biologics) targeting receptor tyrosine kinases (RTKs). This kit assesses RTK activation via the detection of PDGFRb activation-induced SH2 domain protein recruitment. The included cell line overexpresses PK-tagged Human PDGFRb and an EA-tagged SH2 domain protein. Upon ligand (e.g. PDGF-BB)-induced receptor activation, SH2-EA recruitment leads to the production of an EFC signal proportional to the degree of receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

PDGFRbeta

Protein Aliases:

PDGFR-beta|Beta platelet-derived growth factor receptor|Beta-type platelet-derived growth factor receptor|CD140 antigen-like family member B|Platelet-derived growth factor receptor 1|PDGFR-1|CD antigen CD140b

Accession Number:

NM_002609

UniProt Number:

P09619

Gene Name:

PDGFRB

Gene ID:

5159

Gene Aliases:

JTK12|CD140b|PDGFR1|PDGFR

Target Species:

Human

Cell Background:

U2OS