PathHunter® Human MTNR1A Stable Cell Line Total GPCR Internalization Assay (DLD1)

Background Information:

The PathHunter Human MTNR1A Stable Cell Line Total GPCR Internalization Assay (DLD1) utilizes the MTNR1A Total GPCR Internalization cell line. The stable engineered cell line is for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables the assessment of ligand (e.g., melatonin)- based internalization of MTNR1A via the detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged MTNR1A and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, the internalization of the MTNR1A receptor to the early endosome results in the production of the EFC signal. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

GPCR

Family:

Melatonin

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

MT1

Protein Aliases:

Mel1a receptor

Accession Number:

NM_005958

UniProt Number:

P48039

Gene Name:

MTNR1A

Gene ID:

4543

Gene Aliases:

MEL-1A-R

Target Species:

Human

Cell Background:

DLD1