PathHunter® Human IL-2 (IL-2R (IL2RA/IL2RB/IL2RG)) Stable Cell Line Dimerization Assay (U2OS)

Background Information:

The PathHunter Human IL-2 (IL-2R (IL2RA/IL2RB/IL2RG)) Stable Cell Line Dimerization Assay (U2OS) utilizes the IL2RB/IL2RG/IL2RA Dimerization Cell Line. This assay enables the assessment of ligand (e.g. IL-2)-induced assembly of IL2 receptors from IL2RA, IL2RB, and IL2RG subunits. The included cell line employs the PathHunter Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress untagged IL2RA, IL2RB fused to PK, and IL2RG fused to EA, enabling ligand-dependent measurement of receptor complex assembly upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10 plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Cytokine Receptor

Family:

IL-2 Receptor

Protein Name:

IL2RB

Protein Aliases:

IL-2R subunit beta|IL-2RB|High affinity IL-2 receptor subunit beta|Interleukin-15 receptor subunit beta|p70-75|p75|CD antigen CD122

Accession Number:

NM_000878.3

UniProt Number:

P14784

Gene Name:

IL2RB

Gene ID:

3560

Gene Aliases:

IL15RB

Target Species:

Human

Cell Background:

U2OS