PathHunter® Human BTLA Signaling Cell-Based Assay Kit (Jurkat)

Background Information:

The PathHunter® Human BTLA Signaling Cell-Based Assay Kit (Jurkat) utilizes the BTLA Signaling Cell Line (Jurkat). The stable engineered cell line is for use in studying molecules and drug candidates targeting signaling checkpoints. This cell line enables the assessment of BTLA induced non-canonical NF-κB signaling via detection of SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged BTLA target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of a SH2 domain protein to the phosphorylated receptor tail leads to production of the EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Checkpoint Receptor

Protein Name:

BTLA

Accession Number:

NM_181780.3

UniProt Number:

Q7Z6A9

Gene Name:

BTLA

Gene ID:

151888

Gene Aliases:

BTLA1|CD272

Target Species:

Human

Cell Background:

Jurkat