Biological Information

Background Information:

The PathHunter® SSTR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. somatostatin 28) based internalization of SSTR2 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged SSTR2. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting SSTR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Somatostatin

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

sst2

Protein Aliases:

SS2-R|SS2R|SRIF-1

Accession Number:

AY236542

UniProt Number:

P30874

Gene Name:

SSTR2

Gene ID:

6752

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

eXpress Assay Kits

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover

Assay Information

Assay Type:

Cell Based

Assay Measures:

Internalization

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®