PathHunter® eXpress HEK 293 RELA Nuclear Translocation Assay

Background Information:

The PathHunter® eXpress HEK 293 RELA Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the NFκB pathway. This kit assesses ligand (e.g. wortmannin) based pathway activation via detection of resulting RELA nuclear translocation. This product uses EFC technology. The cells included overexpress PL-tagged RELA and nuclear localized EA. Upon pathway activation, induced nuclear translocation of RELA leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Nuclear Protein

Family:

Transcription factor

Protein Name:

RELA

Protein Aliases:

Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|Transcription factor p65

Accession Number:

NM_021975

UniProt Number:

Q04206

Gene Name:

RELA

Gene ID:

5970

Gene Aliases:

NFKB3

Target Species:

Human

Cell Background:

HEK293