PathHunter® eXpress EphB1 Functional Assay

Background Information:

The PathHunter® eXpress EphB1 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated EphB1 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The cells included overexpress PK-tagged EphB1 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

EphB1

Protein Aliases:

ELK|EPH tyrosine kinase 2|EPH-like kinase 6|EK6|hEK6|Neuronally-expressed EPH-related tyrosine kinase|NET|Tyrosine-protein kinase receptor EPH-2

Accession Number:

NM_004441.3

UniProt Number:

P54762

Gene Name:

EPHB1

Gene ID:

2047

Gene Aliases:

Hek6

Target Species:

Human

Cell Background:

U2OS