Biological Information

Background Information:

The PathHunter® CCR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL20/MIP-3α) based activation of CCR6 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged CCR6 and EA-tagged β-Arrestin-2. Activation of CCR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CCR6

Protein Aliases:

CC-CKR-6|CCR-6|Chemokine receptor-like 3|CKR-L3|DRY6|G-protein coupled receptor 29|GPR-CY4|GPRCY4|LARC receptor|CD antigen CD196

Accession Number:

AY242126

UniProt Number:

P51684

Gene Name:

CCR6

Gene ID:

1235

Gene Aliases:

CKR-L3|GPR-CY4|CMKBR6|GPR29|DRY-6|DCR2|BN-1|CD196

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

eXpress Assay Kits

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®