PathHunter® eXpress CCR10 Activated GPCR Internalization Assay

Background Information:

The PathHunter® CCR10 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. CCL27/CTACK) based internalization of CCR10 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged CCR10. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting CCR10-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CCR10

Protein Aliases:

CC-CKR-10|CCR-10|G-protein coupled receptor 2

Accession Number:

AY429103

UniProt Number:

P46092

Gene Name:

CCR10

Gene ID:

2826

Target Species:

Human

Cell Background:

U2OS