PathHunter® EDG7 Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® EDG7 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. LPA) based internalization of EDG7 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged EDG7. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting EDG7-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Lysophospholipid (LPA)

Sub Family:

Class A

Coupling:

Gq

Protein Name:

LPA3

Protein Aliases:

LPA-3|EDG7|Lysophosphatidic acid receptor Edg-7

Accession Number:

NM_012152.2

UniProt Number:

Q9UBY5

Gene Name:

LPAR3

Gene ID:

23566

Gene Aliases:

LP-A3|Edg-7|RP4-678I3|HOFNH30|LPA3

Target Species:

Human

Cell Background:

U2OS