LPA2 (EDG4) Gq Calcium Signaling Cell-Based Assay Kit (CHO-K1)

Background Information:

The LPA2 (EDG4) Gq Calcium Signaling Cell-Based Assay Kit (CHO-K1) utilizes the EDG4 Gq cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) that target GPCRs. This cell line enables the assessment of ligand (e.g., LPA) based activation of EDG4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses EDG4, a naturally Gq coupled GPCR. Upon activation of EDG4, the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Lysophospholipid (LPA)

Sub Family:

Class A

Coupling:

Gq

Protein Name:

LPA2

Protein Aliases:

LPA-2|EDG4|Lysophosphatidic acid receptor Edg-4

Accession Number:

NP_004711

UniProt Number:

Q9HBW0

Gene Name:

LPAR2

Gene ID:

9170

Gene Aliases:

EDG-4|LPA2

Target Species:

Human

Cell Background:

CHO-K1