Biological Information

Background Information:

The FFAR1 (GPR40) Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. FFAs) based activation of FFAR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR1 , a naturally Gq coupled GPCR. Upon activation of FFAR1 , the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Free Fatty Acid

Sub Family:

Class A

Coupling:

Gq

Protein Name:

FFA1 (GPR40)

Accession Number:

NM_005303.2

UniProt Number:

O14842

Gene Name:

FFAR1

Gene ID:

2864

Gene Aliases:

FFA1R

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

Calcium Flux

Detection Method:

Chemiluminescence

Additional Information

Brand:

Eurofins DiscoverX®