Biological Information
Background Information:
The F2RL1 Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serine proteases, SLIGRL-NH2) based activation of F2RL1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses F2RL1 , a naturally Gq coupled GPCR. Upon activation of F2RL1 , the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Proteinase-Activated
Sub Family:
Class A
Coupling:
Gi/Go & Gq
Protein Name:
PAR2
Protein Aliases:
proteinase-activated receptor-2
Accession Number:
NM_005242.4
UniProt Number:
P55085
Gene Name:
F2RL1
Gene ID:
2150
Gene Aliases:
PAR2|GPR11
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
Calcium Flux
Detection Method:
Chemiluminescence
Additional Information
Brand:
Eurofins DiscoverX®