ChemiSCREEN™ MRGPRD Receptor Stable Cell Line

Background Information:

The ChemiSCREEN™ MRGPRD Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. β-alanine) based activation of MRGPRD GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MRGPRD alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of MRGPRD , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Class A Orphan

Sub Family:

Orphan and other 7TM

Protein Name:

MrgD

Protein Aliases:

G-protein coupled receptor TGR7

Accession Number:

NM_198923

UniProt Number:

Q8TDS7

Gene Name:

MRGPRD

Gene ID:

116512

Gene Aliases:

mrgD

Target Species:

Human

Cell Background:

Chem-1