ChemiBRITE EP2 Prostanoid Receptor Stable Cell Line

Background Information:

The ChemiBRITE EP2 Prostanoid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER2 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBRITE cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER2 , the resulting calcium activity can be assayed in either detection mode.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Protein Name:

EP2

Protein Aliases:

PGE2 receptor EP2 subtype|Prostanoid EP2 receptor

Accession Number:

NM_000956

UniProt Number:

P43116

Gene Name:

PTGER2

Gene ID:

5732

Gene Aliases:

EP2

Target Species:

Human

Cell Background:

HEK293