Biological Information

Background Information:

The cAMP Hunter™ LTB4R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. leukotriene B4) based activation of LTB4R GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type LTB4R, a naturally Gi/Go coupled GPCR. Upon activation of LTB4R, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Leukotriene

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

BLT1 (LTB4)

Protein Aliases:

LTB4-R1|Chemoattractant receptor-like 1|G-protein coupled receptor 16|P2Y purinoceptor 7|P2Y7

Accession Number:

AY322535

UniProt Number:

Q15722

Gene Name:

LTB4R

Gene ID:

1241

Gene Aliases:

BLT|BLT1|BLTR|CMKRL1|GPR16|P2RY7

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

eXpress Assay Kits

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover

Assay Information

Assay Type:

Cell Based

Assay Measures:

cAMP Detection

Detection Method:

Chemiluminescence

Additional Information

Brand:

cAMP Hunter™