cAMP Hunter™ CHO-K1 GPBAR1 Bioassay Kit

Background Information:

The cAMP Hunter™ CHO-K1 GPBAR1 Bioassay Kit provides a robust and highly sensitive functional, cell-based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. TGR5 receptor agonist) based activation of GLP-1R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP-1R, a naturally Gs coupled GPCR. Upon activation of GLP-1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Bile Acid

Sub Family:

Class A

Protein Name:

TGR5

Protein Aliases:

hGPCR19|Membrane-type receptor for bile acids|M-BAR|hBG37|BG37

Accession Number:

NM_001077194.1

UniProt Number:

Q8TDU6

Gene Name:

GPBAR1

Gene ID:

151306

Gene Aliases:

BG37|GPCR|TGR5|M-BAR|GPCR19|GPR131|MGC40597

Target Species:

Human

Cell Background:

CHO-K1