cAMP Hunter™ CHO-K1 CRTH2 Gi Cell Line

Background Information:

The cAMP Hunter™ CRTH2 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin D2) based activation of CRTH2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CRTH2 , a naturally Gi/Go coupled GPCR. Upon activation of CRTH2 , the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

DP2

Protein Aliases:

chemoattractant receptor homologous molecule expressed on T helper type 2 cells|G-protein coupled receptor 44|CD antigen CD294

Accession Number:

AY507142

UniProt Number:

Q9Y5Y4

Gene Name:

PTGDR2

Gene ID:

11251

Gene Aliases:

CRTH2|CD294|DP2|DL1R|GPR44

Target Species:

Human

Cell Background:

CHO-K1