Ready-to-Assay S1P1 Lysophospholipid Receptor Frozen Cells

Background Information:

The Ready-to-Assay S1P1 Lysophospholipid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. S1P) based activation of S1PR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress S1PR1 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of S1PR1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Lysophospholipid (S1P)

Sub Family:

Class A

Protein Name:

S1P1

Protein Aliases:

EDG1|Endothelial differentiation G-protein coupled receptor 1|Sphingosine 1-phosphate receptor Edg-1|S1P receptor Edg-1|CD antigen CD363

Accession Number:

NM_001400

UniProt Number:

P21453

Gene Name:

S1PR1

Gene ID:

1901

Gene Aliases:

edg-1|D1S3362|CD363

Target Species:

Human

Cell Background:

Chem-1