Ready-to-Assay MCH2 Melanin-Concentrating Hormone Receptor Frozen Cells

Background Information:

The Ready-to-Assay MCH2 Melanin-Concentrating Hormone Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. MCH) based activation of MCHR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress MCHR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MCHR2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Melanin-Concentrating Hormone

Sub Family:

Class A

Protein Name:

MCH2

Protein Aliases:

MCH-R2|MCHR-2|G-protein coupled receptor 145|GPRv17|MCH-2R|MCH2|MCH2R

Accession Number:

AY562946

UniProt Number:

Q969V1

Gene Name:

MCHR2

Gene ID:

84539

Gene Aliases:

SLT|MCH2|MCH2R

Target Species:

Human

Cell Background:

Chem-1