Ready-to-Assay CysLT1 Leukotriene Receptor Frozen Cells

Background Information:

The Ready-to-Assay CysLT1 Leukotriene Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. leukotriene) based activation of CysLT1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CysLT1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of CysLT1 , the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Leukotriene

Sub Family:

Class A

Protein Name:

CysLT1

Accession Number:

NM_006639

UniProt Number:

Q9Y271

Gene Name:

CYSLTR1

Gene ID:

10800

Gene Aliases:

CysLT1|CysLT(1)|CYSLT1R

Target Species:

Human

Cell Background:

HEK293