Ready-to-Assay CXCR5 Chemokine Receptor Frozen Cells

Background Information:

The Ready-to-Assay CXCR5 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CXCL13/BCA-1/BLC) based activation of CXCR5 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CXCR5 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CXCR5 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Protein Name:

CXCR5

Protein Aliases:

CXCR-5|Burkitt lymphoma receptor 1|Monocyte-derived receptor 15|MDR-15|CD antigen CD185

Accession Number:

NM_001716

UniProt Number:

P32302

Gene Name:

CXCR5

Gene ID:

643

Gene Aliases:

MDR15|CD185

Target Species:

Human

Cell Background:

Chem-10