Biological Information

Background Information:

The PathHunter® mPD-L1 Ligand cell line is a stable, engineered cell line designed for use with mPD-1 Checkpoint Signaling Cell Lines. When co-cultured, the mPD-L1 expressed in this cell line will induce activity of mPD-1 receptors, which may be assayed through SH2 domain protein recruitment. This product is a companion product designed to work with Immunoglobulin Superfamily Checkpoint Assays. The cell line included overexpresses mPD-L1 cell surface ligand. When co-cultured with the corresponding Checkpoint Signaling Cell Line, the mPD-L1 ligand binds to the checkpoint receptor, inducing receptor activity. This receptor activity leads to recruitment of the relevant SH2 domain protein to the checkpoint receptor inside the receptor cell line and production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells. Please note this product expresses the mouse version of PD-L1.

Target Class:

Checkpoint Receptor

Family:

IgSF

Sub Family:

Immune Inhibitory Receptor Ligand

Protein Name:

PD-L1

Protein Aliases:

PDCD1 ligand 1|CD274 molecule|B7 homolog 1|B7-H1|CD antigen CD274

Accession Number:

NM_021893

UniProt Number:

Q9EP73

Gene Name:

Cd274

Gene ID:

60533

Gene Aliases:

B7h1|Pdl1|Pdcd1l1|Pdcd1lg1|A530045L16Rik

Target Species:

Mouse

Cell Background:

U2OS

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

Signaling

Detection Method:

Chemiluminescence

Clinical Relevance

Therapeutic Area:

Oncology/Immuno-Oncology

Additional Information

Brand:

PathHunter®