PathHunter® EBI2 Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® EBI2 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. S1P) based internalization of EBI2 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged EBI2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the EBI2 receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Class A Orphan

Sub Family:

Orphan and other 7TM

Coupling:

Gi/Go

Protein Name:

GPR183

Protein Aliases:

EBI2|EBV-induced G-protein coupled receptor 2|hEBI2

Accession Number:

NM_004951.4

UniProt Number:

P32249

Gene Name:

GPR183

Gene ID:

1880

Gene Aliases:

EBI2

Target Species:

Human

Cell Background:

U2OS