Biological Information
Background Information:
The PathHunter® EDG7 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. LPA) based activation of EDG7 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged EDG7 and EA-tagged β-Arrestin 2. Activation of EDG7 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Lysophospholipid (LPA)
Sub Family:
Class A
Coupling:
Gq
Protein Name:
LPA3
Protein Aliases:
LPA-3|EDG7|Lysophosphatidic acid receptor Edg-7
Accession Number:
NM_012152.2
UniProt Number:
Q9UBY5
Gene Name:
LPAR3
Gene ID:
23566
Gene Aliases:
LP-A3|Edg-7|RP4-678I3|HOFNH30|LPA3
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®