Biological Information

Background Information:

The InCELL Hunter™ A549 PYK2 Kinase Binding Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of PYK2 via detection of PYK2 levels. This product uses EFC technology. The cell line included overexpresses PYK2 target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Kinase

Family:

TK

Sub Family:

Protein Tyrosine

Protein Name:

PYK2

Protein Aliases:

Calcium-dependent tyrosine kinase|CADTK|Calcium-regulated non-receptor proline-rich tyrosine kinase|Cell adhesion kinase beta|CAK-beta|CAKB|Focal adhesion kinase 2|FADK 2|Proline-rich tyrosine kinase 2|Related adhesion focal tyrosine kinase|RAFTK

Accession Number:

NC_001147.6

UniProt Number:

Q14289

Gene Name:

PTK2B

Gene ID:

2185

Gene Aliases:

CAKB|PYK2|RAFTK|PTK|CADTK|FAK2

Target Species:

Human

Cell Background:

A549

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based Binding

Assay Measures:

Target Engagement

Detection Method:

Chemiluminescence

Clinical Relevance

Therapeutic Area:

Oncology/Immuno-Oncology

Additional Information

Brand:

InCELL Hunter™