Ubiquitination Proteins

Recombinant Active, Purified E1, E2, E3 and Substrate Proteins for Screening, Structural Analysis, and Mechanism of Action Studies

Ubiquitination is a highly orchestrated post-translational modification where the ubiquitin polypeptide is covalently conjugated to target proteins through an enzyme-dependent cascade. This multilayered and dynamically regulated process governs a wide spectrum of cellular activities, including proteasomal degradation, cell-cycle progression, DNA damage recognition, and repair. Ubiquitination also functions as a central component of the protein quality-control machinery, ensuring the removal of misfolded or damaged proteins. Perturbations in ubiquitin pathway homeostasis are strongly associated with the pathogenesis of multiple disorders, notably cancer and neurodegenerative diseases. As a result, components of the ubiquitination cascade have emerged as compelling targets for therapeutic intervention and drug development.

Eurofins DiscoverX provides a wide range of recombinant proteins designed to facilitate research programs that explore ubiquitination. These validated recombinant ubiquitination cascade components, including functionally active E1, E2, and E3 enzymes, allow you to study and manipulate the ubiquitin system in vitro with high confidence and reproducibility.

Partner with us to bring high-quality, pre-clinical–ready ubiquitination biochemistry into your laboratory and accelerate your path from discovery to therapeutic development.

Product Highlights
  • Drug Discovery Applications — Ideal for exploring E3 ligases, developing PROTAC®s, or profiling ubiquitination in disease-relevant pathways
  • Validated, Ubiquitination Cascade-ready Reagents — All proteins are functionally tested in full E1→E2→E3→substrate ubiquitination reactions, giving you a research-ready platform rather than untested reagents
  • High Reproducibility and Lot-to-lot Consistency — Minimizes assay variability, improves data quality and reproducibility across experiments and laboratories
  • Scalable and Flexible — Suitable for low-throughput mechanistic biochemistry or high-throughput screening (HTS) workflows

Consider Eurofins DiscoverX’s custom development capabilities for custom cell lines, assays, & enzyme development.

PROTAC® is a registered trademark of Arvinas.

SHOP PRODUCTS

Highlights

Applications

  • E1, E2, E3 screening — Perform biochemical screening of small molecules affecting ubiquitination.
  • Targeted Protein Degradation (TPD) — Use our E3 ligase + substrate pairs to model and validate PROTAC efficacy in vitro before cellular studies.
  • Mechanistic biochemistry — Study kinetics, substrate specificity, E2–E3 pairing, influence of disease-relevant mutations, or regulatory modifications.
  • Chain linkage and topology studies — Define which ubiquitin chain linkages are formed by specific E2–E3-substrate combinations; critical for degraders or signalling research.

Benefits

  • Full-length, functionally active purified E1, E2 and E3 enzymes — Guarantees authentic protein:protein interactions and permits study of allosteric inhibitors.
  • Validated ubiquitination functionality — All proteins undergo functional validation within complete E1→E2→E3→substrate ubiquitination assays, ensuring that you receive a fully characterized, research-ready system rather than unverified individual components.

Product Portfolio

  • Large panels of functionally active, purified E3 ligase enzymes – Encompassing many of the most widely studied enzymes such as VHL, SCFSkp2, SCFβTRCP1, SCFFBW7, and CRBN.
  • Need a custom E3 ligase or substrate not listed? Visit custom protein production and contact us for your request.

Credibility

  • Recognized by leading pharma and biotech for HTS, lead optimization, and mechanism of action (MOA) studies.
  • Supported by extensive technical documentation and scientific expertise.
  • Helpful and experienced customer support.