Build Your Own Assay Tools

Complete Set of Tools to Build Your Own Cell Lines and Cell-Based Assays

Do-It-yourself toolbox products allow you to develop in-house cell lines and cell-based assays for your target and application of interest. Obtain the benefits of using optimized toolbox products for creating your ideal cell line or assay to save you the effort and time required for assay development.

Take advantage of Eurofins DiscoverX®'s proprietary Enzyme Fragment Complementation (EFC) technology to develop your own stable cell lines and cell-based assays to study protein-protein interactions, compound-target engagement, cytotoxicity, receptor dimerization and signaling, protein translocation, and much more.

Gain an in-depth understanding of the assay principle for consideration of creating optimized assays for multiple applications. Reach out to scientific experts to guide your experimental design through analysis of your results. Design your workflows and utilize engineered parental cell lines, retroparticles, and plasmid vectors to introduce your target of interest and create cell-based assays for multiple applications.

Product Highlights
  • Simple Protocols – Detailed, step-by-step instructions with flow-charts and infographics to easily create assays
  • Flexible Solutions – Utilize any of our cell-line expression vectors to introduce mutants, make modifications, or study unique orthologs or isotypes
  • Any Cell Background – Make your own stable β-arrestin recruitment or cytotoxicity cell-based assays in any dividing cell type with PathHunter® or KILR® retroparticles
  • Complete Kit - Ready-to-use InCELL Pulse™ starter kit includes all the reagents you need to get you started to create your own target engagement assays for your target of interest
SHOP PRODUCTSPARENTAL CELL LINES

Products

Toolbox Products

  • PathHunter ProLink™ and ProLabel® Cloning Vectors – Utilize pCMV Mammalian cloning vectors to clone in your protein of interest with a ProLink or ProLabel small β-galactosidase enzyme donor fragment, as an N- or C-terminal tag, and subsequently transfecting into a cell line to create Enzyme Fragment Complementation (EFC) cell-based assays.
  • Expression Vectors – Introduce an expression vector into unique cell types to create EFC cell-based assays. These vectors include a protein target found in our Eurofins DiscoverX cell-based assays tagged with a ProLink or ProLabel sequence.
  • PathHunter Parental Cell Lines – Utilize engineered PathHunter parental cells to rapidly develop β-arrestin recruitment, SH2-protein recruitment, receptor dimerization, or translocation EFC cell-based assays for your protein of interest. These parental cell lines provide the ability to study β-arrestin recruitment with your favorite GPCR, analyze kinase or cytokine receptor dimerization and signaling through SH2-recruitment, or investigate trafficking of your target protein to the plasma membrane, nucleus, or endosome.
  • ChemiSCREEN™ or ChemiBRITE™ Parental Cell Lines – Generate your own stable GPCR cell lines for monitoring calcium or cAMP accumulation. Use with chemiluminescent or fluorescent assays to analyze agonists, antagonists, and modulators of your GPCR target to study functional response. Ready-to-Assay parental control frozen cells are also available.
  • PathHunter β-Arrestin Retroparticles – Make your own stable β-arrestin cell lines and EFC cell-based assays for your target in any dividing cell type. Use the assays to analyze the activation and desensitization of GPCRs and regulation of other signaling molecules such as protein kinases that involve β-arrestin recruitment.
  • KILR Retroparticles – Generate target cells expressing native or engineered surface antigens of choice, in the chosen dividing cell, to measure target cell death in co-cultures. These designer cytotoxicity assays allow you to study antibody dependent cell-mediated cytotoxicity (ADCC), complement dependent cytotoxicity (CDC), antibody dependent cell phagocytosis (ADCP), and other cytotoxicity applications.
  • InCELL Pulse Target Engagement Starter Kit – Easily make your own Compound-Target Engagement cell-based assays to measure inhibitor cellular potency against user-defined protein targets of interest. These assays apply EFC and ligand-dependent thermal stabilization of target proteins such as protein kinases, methyltransferases, and hydrolases. Employ the simple protocol with minimal hands-on time to obtain quantitative inhibitor EC50 values in a native cellular environment.