Ready-to-Assay CysLT2 Leukotriene Receptor Frozen Cells

Background Information:

The Ready-to-Assay CysLT2 Leukotriene Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. leukotriene) based activation of CYSLTR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CYSLTR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CYSLTR2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Leukotriene

Sub Family:

Class A

Protein Name:

CysLT2

Protein Aliases:

G-protein coupled receptor GPCR21|hGPCR21|G-protein coupled receptor HG57|HPN321

Accession Number:

NM_020377

UniProt Number:

Q9NS75

Gene Name:

CYSLTR2

Gene ID:

57105

Gene Aliases:

CysLT(2)|CYSLT2R|CYSLT2|PSEC0146

Target Species:

Human

Cell Background:

Chem-1