PTGER1 Gq Stable Cell Line Calcium Signaling Assay (CHO-K1)

Background Information:

The PTGER1 Gq Stable Cell Line Calcium Signaling Assay (CHO-K1) utilizes the PTGER1 Gq cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) that target GPCRs. This cell line enables the assessment of ligand (e.g., prostaglandin E2) based activation of PTGER1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER1, a naturally Gq coupled GPCR. Upon activation of PTGER1, the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Coupling:

Gq

Protein Name:

EP1

Protein Aliases:

PGE2 receptor EP1 subtype|Prostanoid EP1 receptor

Accession Number:

NM_000955.2

UniProt Number:

P34995

Gene Name:

PTGER1

Gene ID:

5731

Gene Aliases:

EP1

Target Species:

Human

Cell Background:

CHO-K1