PathHunter® Human RANKL (RANK) Signaling Reporter Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human RANKL (RANK) Signaling Reporter Cell-Based Assay Kit (U2OS) utilizes the U2OS RANK NF-κB Signaling Pathway Reporter cell line. This engineered cell line monitors the activation of specific signaling pathways, utilizing the established PathHunter Enzyme Fragment Complementation (EFC) technology. It can be used for studying drug candidates (small molecules or biologics) affecting the NF-kB pathway. This cell line enables assessment of NF-kB pathway stimulation based on changes in the expression of an NF-kB response element-driven reporter protein. The included cell line overexpresses RANK, and expresses PL-tagged reporter protein under the control of a NF-kB-inducible transcriptional response element. Pathway activation induces expression of the PL-tagged protein, allowing detection of the EFC signal upon addition of the EA-containing detection reagent.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Signaling Pathway

Family:

Tumour Necrosis Factor (TNF)

Protein Name:

RANK

Protein Aliases:

ODFR|TNF receptor superfamily member 11a|CD antigen CD265

Accession Number:

NM_003839.3; NM_003998.4

UniProt Number:

Q9Y6Q6

Gene Name:

TNFRSF11A

Gene ID:

8792

Gene Aliases:

RANK

Target Species:

Human

Cell Background:

U2OS