PathHunter® Human NF-kB (RELA)-IkB Nuclear Translocation Cell-Based Assay Kit (HEK 293)

Background Information:

The PathHunter® Human NF-kB (RELA)-IkB Nuclear Translocation Cell-Based Assay Kit (HEK 293) utilizes the HEK 293 RELA Nuclear Translocation cell line. The stable engineered cell line is for use in studying drug candidates that affect the NFκB pathway. This cell line enables assessment of ligand (e.g. wortmannin) based pathway activation via detection of resulting RELA nuclear translocation. This product uses EFC technology. The included cell line overexpresses PL-tagged RELA (p65) and nuclear localized EA. Upon pathway activation, induced nuclear translocation of RELA leads to production of the EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Nuclear Protein

Family:

Transcription factor

Protein Name:

RELA

Protein Aliases:

Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|Transcription factor p65

Accession Number:

NM_021975

UniProt Number:

Q04206

Gene Name:

RELA

Gene ID:

5970

Gene Aliases:

NFKB3

Target Species:

Human

Cell Background:

HEK293