PathHunter® Human IκB Degradation Cell-Based Assay Kit (A549)

Background Information:

The PathHunter® Human IκB Degradation Cell-Based Assay Kit (A549) utilizes the PathHunter® A549 IκB Degradation cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) targeting the NFκB pathway. This cell line enables the detection of IκB levels, allowing for the assessment of NFκB pathway activation-induced IκB degradation. This product uses EFC technology. The cell line included overexpresses IκB target protein fused to PL. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of IκB, the EFC signal is lost.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Signaling Pathway

Protein Name:

IKBA

Protein Aliases:

IkB-alpha|IkappaBalpha|Major histocompatibility complex enhancer-binding protein MAD3

Accession Number:

NM_020529

UniProt Number:

P25963

Gene Name:

NFKBIA

Gene ID:

4792

Gene Aliases:

IKBA|MAD3|NFKBI

Target Species:

Human

Cell Background:

A549