PathHunter® Human GM-CSF (CSF2RB/CSF2RA) Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human GM-CSF (CSF2RB/CSF2RA) Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS CSF2RB/CSF2RA Dimerization Cell Line. This assay enables the assessment of ligand (e.g. GM-CSF)-induced dimerization of CSF2RB/CSF2RA pairs. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress CSF2RB fused to PK and CSF2RA fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Cytokine Receptor

Family:

IL-3 Receptor

Protein Name:

CSF2RB

Protein Aliases:

Colony stimulating factor 2 receptor beta common subunit

Accession Number:

NM_000395.2

UniProt Number:

Q6NSJ8

Gene Name:

CSF2RB

Gene ID:

1439

Target Species:

Human

Cell Background:

U2OS