PathHunter® Human AHR Nuclear Receptor Protein Interaction Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human AHR Nuclear Receptor Protein Interaction Cell-Based Assay Kit (U2OS) utilizes the ARH Protein Interaction cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. FICZ) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged ARH NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of ARH, induced interaction with SRCP leads to production of the EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

NHR

Family:

Aryl Hydrocarbon

Sub Family:

Non-Steroid

Protein Name:

AHR

Protein Aliases:

AhR|Class E basic helix-loop-helix protein 76|bHLHe76

Accession Number:

NM_001621.4

UniProt Number:

P35869

Gene Name:

AHR

Gene ID:

196

Gene Aliases:

bHLHe76

Target Species:

Human

Cell Background:

U2OS