mGlu5 (GRM5) Gq Calcium Signaling Cell-Based Assay Kit (U2OS)

Background Information:

The mGlu5 (GRM5) Gq Calcium Signaling Cell-Based Assay Kit (U2OS) utilizes the GRM5 Gq cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) that target GPCRs. This cell line enables the assessment of ligand (e.g., L-glutamate) based activation of GRM5 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GRM5, a naturally Gq coupled GPCR. Upon activation of GRM5, the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

GPCR

Family:

Glutamate (Metabotropic)

Sub Family:

Class C

Coupling:

Gq

Protein Name:

mGlu5

Protein Aliases:

protein phosphatase 1, regulatory subunit 86

Accession Number:

NM_000842.3

UniProt Number:

P41594

Gene Name:

GRM5

Gene ID:

2915

Gene Aliases:

MGLUR5|GPRC1E|mGlu5|PPP1R86

Target Species:

Human

Cell Background:

U2OS