InCELL Hunter™ Human G9a Stable Cell Line Target Engagement Assay (A549)

Background Information:

The InCELL Hunter Human G9a Stable Cell Line Target Engagement Assay (A549) utilizes the G9a Target Engagement cell line. The stable engineered cell line is intended for use in studying small molecule drug candidates that engage with regulators of epigenetic pathways. This cell line enables assessment of compound-engagement-based stabilization of G9a via detection of G9a levels. This product uses EFC technology. The cell line included overexpresses G9a's methyltransferase domain fused to PL. In the absence of a compound that binds and stabilizes the target, this protein fragment is naturally degraded and no EFC signal is produced. When the compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Epigenetic

Family:

Histone Methyltransferase (HMT)

Sub Family:

Writer

Protein Name:

G9A

Protein Aliases:

Euchromatic histone-lysine N-methyltransferase 2|HLA-B-associated transcript 8|Histone H3-K9 methyltransferase 3|H3-K9-HMTase 3|Lysine N-methyltransferase 1C|Protein G9a

Accession Number:

NM_006709

UniProt Number:

Q96KQ7

Gene Name:

EHMT2

Gene ID:

10919

Gene Aliases:

G9A|Em:AF134726.3|NG36/G9a|KMT1C

Target Species:

Human

Cell Background:

A549