cAMP Hunter™ Human MC4R Gs Cell-Based Assay Kit (CHO-K1)

Background Information:

The cAMP Hunter™ Human MC4R Gs Cell-Based Assay Kit (CHO-K1) utilizes the Human MC4R cAMP cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of cAMP signaling following receptor activation for drug discovery and development programs. This cell line enables assessment of ligand (e.g. α-melanocyte stimulating hormone) based activation of MC4R GPCR activity via detection of increasing levels of intracellular cAMP. This assay utilizes EFC technology to measure ligand-induced activation by quantifying intracellular cAMP levels. The cells overexpress wild type MC4R, a naturally Gs coupled GPCR. Upon activation of MC4R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This product leverages a sensitive chemiluminescent cAMP detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Melanocortin

Sub Family:

Class A

Coupling:

Gs

Protein Name:

MC4

Accession Number:

NM_005912.2

UniProt Number:

P32245

Gene Name:

MC4R

Gene ID:

4160

Target Species:

Human

Cell Background:

CHO-K1